We know from looking at the Lineweaver-Burk equation that is the slope of the line. In problem 3, we determined that the V_max of this enzyme is 50.

Using this information, we can write,

By visually inspecting the graph we can see that points,

lie on the graph of the line. Thus, we find the slope of the line to be,

Solving for K_m in the above equation yields,

K_m = (50)(0.5) = 25 µM

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